SAM #Solicitation1232SA26Q1506

USDA ARS NCWMAC Sea Lice DNA Extraction and Genotyping Services (AXIOM LSAL200K 96-ARRAY)

This opportunity from the USDA Agricultural Research Service's National Cold Water Marine Aquaculture Center (NCWMAC) focuses on sea lice DNA extraction and genotyping services. - Government Buyer: - USDA Agricultural Research Service - National Cold Water Marine Aquaculture Center (NCWMAC) - OEMs and Vendors: - Thermo-Fisher Scientific (OEM for AXIOM LSAL200K 96-ARRAY genotyping platform) - Products/Services Requested: - DNA extraction and SNP genotyping for sea lice (Lepeophtheirus salmonis) - Use of AXIOM LSAL200K 96-ARRAY genotyping (Thermo-Fisher SKU 550522) in 96-well format - Processing of 960 plates - Genotyping performed on GeneTitan Instrument or equivalent system - Delivery of genotyping data and raw files in CEL and PLINK formats - Return of unused tissue and DNA samples to the government - Unique/Notable Requirements: - Quality control and sample tracking via Laboratory Information Management System (LIMS) - Data delivery within 60 days - Contractor must provide all necessary services, supplies, equipment, and facilities - Total small business set-aside; firm-fixed price anticipated - Thermo-Fisher is the only OEM specifically referenced

Description

The USDA Agricultural Research Service requires DNA extraction from sea lice (Lepeophtheirus salmonis) and single nucleotide polymorphism (SNP) genotyping.  Lice tissue samples will consist of single pre-adult or adult animals that have been preserved in ethanol.   Sea lice DNA genotyping will be completed using AXIOM LSAL200K 96-ARRAY genotyping in the 96-well format (Thermo-Fisher SKU number 550522) on the GeneTitan Instrument or equal. Deliverables will include the genotyping data for all the samples and the raw data files in .cel format (CEL files) and PLINK format (either .ped and .map or .bed, .bim, and .fam).  Unused tissue samples and DNA samples will be sent back to the government.  Contractor shall provide all services, supplies, equipment and facilities necessary to provide the deliverable specified in accordance with the terms, conditions, and specifications contained in this document.         

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