SAM #127EAV26Q0125

Wheat DNA Library Sequencing Services for USDA Plant Science Research Unit

This opportunity from the USDA Agricultural Research Service, Plant Science Research Unit in Raleigh, NC, seeks genomic sequencing services for wheat DNA libraries to support plant genetics research. - Government Buyer: - United States Department of Agriculture (USDA), Agricultural Research Service - Plant Science Research Unit, Raleigh, NC - Office: USDA-FS CSA Southwest 5 - OEMs and Vendors: - Element Biosciences (AVITI platform specified; equivalent platforms accepted) - Products/Services Requested: - Quality control of 22 wheat DNA library pools - Qubit or equivalent fluorometric quantification - Tapestation analysis - qPCR validation - Sequencing services using Element Biosciences AVITI or equivalent platform - 2 paired-end high-flow-cell sequencing runs (2 x 150, 1 billion read pairs each) - 10 single-end high-flow-cell sequencing runs (1 x 150, 1 billion reads each) - Deliverables: FASTQ files and quality reports within 21 days of sample receipt - Unique/Notable Requirements: - Sequencing must meet minimum industry standards for library QC - All sequencing must be performed on AVITI or equivalent platforms - Firm-fixed-price contract, total small business set-aside - Delivery required within 365 days - Minimum yield and base pair requirements per flow cell - Data delivery in FASTQ format with quality reports

Description

The United States Department of Agriculture, Agricultural Research Service, Plant Science Research Unit, Raleigh, North Carolina requires AVITI Next Generating Sequencing of wheat DNA libraries. The resulting data will be analyzed and utilized in conjunction with the Plant Science Research Unit’s ongoing genetic investigation of complex traits of plants.

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