SAM #12444726Q0077

USDA Genotyping and Sequencing Services for Tropical-Derived Corn Lines (GEM Project)

This opportunity is for specialized genotyping and sequencing services to support the USDA Germplasm Enhancement of Maize (GEM) Project. - Government Buyer: - USDA Forest Service, CSA EAST 8 office - USDA Germplasm Enhancement of Maize Project, North Central Regional Plant Introduction Station - USDA Agricultural Research Service (ARS), MWA Plant Introduction Research, Iowa State University - OEM/Vendor: - Mol Breeding Biotechnology LLC (sole-source provider) - Products/Services Requested: - Genotyping and sequencing services for tropical-derived corn lines - Probe-in-solution target enrichment - Kernel chipping and DNA extraction - Advanced library preparation (fragmentation, end repair, A-tail addition, adapter ligation, library amplification with barcode primers) - High-quality sequencing and quality control - Comprehensive bioinformatics analysis with user-friendly interface - Use of proprietary, validated SNP panels (~45,000 primary SNPs and ~10,000 secondary SNPs) - Reference laboratory testing for 10,000 and 45,000 SNP panels - Unique/Notable Requirements: - Only Mol Breeding Biotechnology LLC can provide the required proprietary SNP panels and integrated sequencing-to-analysis workflow - Continuity with existing GEM datasets is essential - Corn seeds will be furnished by the government - Services must be completed within 180 days of seed receipt - Supports genomic selection for disease resistance, drought tolerance, and pest resistance in maize - Compliance with federal anti-discrimination and DEI policies is required

Description

The USDA intends to award a sole‑source, firm‑fixed‑price contract to Mol Breeding Biotechnology LLC for targeted sequencing and genotyping of tropical‑derived corn lines under the Germplasm Enhancement of Maize (GEM) Project. The requirement includes probe‑in‑solution target enrichment, kernel chipping, DNA extraction, advanced library preparation, high‑quality sequencing, and comprehensive bioinformatics analysis using proprietary, validated SNP panels (~45,000 primary SNPs and ~10,000 secondary SNPs). These panels are essential for maintaining continuity with existing GEM datasets and can only be provided through MOLBREEDING BIOTECHNOLOGY LLC’s specialized workflows.

Due to the unique technical tools, germplasm‑validated markers, and integrated sequencing‑to‑analysis processes required, the Government has determined that this requirement can only be met by MOLBREEDING BIOTECHNOLOGY LLC within the 180‑day performance period. Capability statements from other vendors will be reviewed solely to assess whether competitive procurement is feasible. The decision to compete the requirement rests solely with the contracting officer, and no reimbursement will be provided for the submission of capability information.

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