SAM #75N98026Q00916

NIH Procurement of Bio-Rad QX600 Droplet Digital PCR System and Deepwell Thermal Cycler

NIH is seeking advanced laboratory equipment for nucleic acid quantification and PCR analysis. - Government Buyer: - National Institutes of Health (NIH), Office of Logistics and Acquisition Operations (OLAO), Department of Health and Human Services - OEM Highlight: - Bio-Rad Laboratories is the sole specified manufacturer - Products Requested: - One (1) Bio-Rad QX600 Droplet Digital PCR System - Provides absolute nucleic acid quantification - Features six-color multiplex fluorescence detection - Processes up to 96 samples per run - Includes all required computer hardware and system software for automated thresholding, Poisson analysis, multiplex analysis, and result export - One (1) Bio-Rad PTC Harmony Deepwell Thermal Cycler - Compatible with 96-well deepwell PCR plates - Unique Requirements: - No user training required - Equipment must match Bio-Rad's published technical specifications - Software must support automated analysis and export - Vendors: - Only Bio-Rad Laboratories is mentioned; no resellers or integrators specified - Notable Technical Features: - High sensitivity and multiplex capability for ddPCR - Direct quantification without standard curves - System designed for high-throughput laboratory workflows

Description

                                                                         Requirements

The laboratory requires a digital droplet PCR (ddPCR) platform capable of absolute nucleic acid quantification with high sensitivity and multiplex capability. 

The quoted system provides up to six-color multiplex detection, processes up to 96 samples per run, and performs absolute nucleic acid quantification 

Type of Order:  

Firm Fixed Price Purchase Order 

Special Order Requirements:  

The instrument shall: 

Perform digital droplet PCR using partitioned droplet technology.  

Partition each 20 μL reaction into approximately 20,000 droplets.  

Support six-color fluorescence detection.  

Process 96-well plates.  

Provide absolute nucleic acid quantification without reliance on standard curves.  

Support applications including:  

mutation detection  

copy number variation  

gene expression  

viral titer analysis  

Include software capable of automated thresholding, Poisson analysis, multiplex analysis, and export of results.  

Include all required computer hardware and system software.  

Include a compatible thermal cycler capable of operating 96-well deepwell PCR plates.  

These specifications are consistent with the manufacturer's published technical specifications. 

The contractor shall deliver the following equipment to the National Institutes of Health (NIH): 

One (1) Bio-Rad QX600 Droplet Digital PCR System  

One (1) Bio-Rad PTC Harmony Deepwell Thermal Cycler  

Training:  

Training is not included under this purchase. The contractor is not required to provide user training as part of this award.

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