SAM #36C26126Q1196

Sole Source Single Cell RNA Sequencing Services for VA Palo Alto Health Care System

This notice announces the VA Palo Alto Health Care System's intent to award a sole source contract for advanced single cell RNA sequencing services. - Agency: Department of Veterans Affairs, VA Palo Alto Health Care System (VAPAHCS) - Contracting Office: 261-Network Contract Office 21 - Sole Source Vendor: Novogene Corporation Inc. - Service: Single cell 3 RNA sequencing (scRNA seq) on fresh human cell samples - Includes library preparation, sequencing, quality control, and bioinformatics analysis - Sequencing must use 10x Genomics workflow and Ultima UG100 platform - Target: 400 million raw reads per sample - All work performed at contractor's Sacramento, CA laboratory - Local, same-day sample pickup and processing required to preserve RNA integrity - Secure data return via SFTP - Purpose: Supports advanced transcriptional disease biology research - Estimated contract value: $18,171.00 - Only vendors able to fully match technical and logistical requirements will be considered - OEMs referenced: 10x Genomics, Ultima - Period of performance: One year from date of award

Potential competitors must demonstrate complete equivalence in technical capability, workflow, and sample handling to be considered.

Description

NOTICE OF INTENT TO SOLE SOURCE Department of Veterans Affairs VA Palo Alto Health Care System (VAPAHCS) Acquisition ID#: 36C261 26 AP 4343

The Department of Veterans Affairs, VA Palo Alto Health Care System (VAPAHCS), intends to award a Firm Fixed Price sole source contract to Novogene Corporation Inc. for single cell 3 RNA sequencing (scRNA seq) on fresh human cell samples, including full library preparation, sequencing, quality control, and downstream bioinformatics analysis.

Authority This acquisition will be conducted under FAR 13.106 1(b)(1), soliciting from a single source when the contracting officer determines that only one source is reasonably available.

Description of Requirement VAPAHCS Research Service requires highly specialized 10x Genomics single cell RNA sequencing, including: Local same day pickup and immediate processing of fresh human samples to preserve RNA integrity Sample QC 10x Genomics library preparation Sequencing on the Ultima UG100 platform targeting 400 million raw reads per sample Full bioinformatics pipeline: demultiplexing, alignment, cell calling, clustering, HVG identification, marker gene detection, enrichment analysis Secure data return via SFTP All work performed at contractor s Sacramento, CA laboratory This work supports ongoing transcriptional level disease biology research requiring advanced single cell resolution. Estimated value: $18,171.00 (Firm Fixed Price) Period of Performance One (1) year from date of award, consistent with the supporting justification package.

Not a Request for Quotes This notice is not a request for competitive quotes. However, interested parties may submit capability statements demonstrating they can meet every mandatory technical requirement. Send information to Rachael.Becker@va.gov. Contractors who do not provide the ability to perform the requirements identified below will not be considered responsive. The Government will use responses solely to determine if a competitive opportunity exists.

Response Requirements Respondents must demonstrate: Ability to process fresh human samples same day, locally, without degradation Access to the Ultima UG100 platform Full 10x single cell workflow capability Full bioinformatics workflow Ability to meet the turnaround time and sample handling constraints Proof of relevant authorization and technical capability

Only responses demonstrating complete equivalence will be considered.

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