SAM #NHGRI-09000

NIH sole source procurement of NEB consumables and reagents for plasma exosomal RNA sequencing

This opportunity is a sole source procurement by the NIH Center for Research on Genomics and Global Health for consumables and reagents from New England Biolabs (NEB) to process 650 plasma exosomal RNA samples for next generation sequencing on the Illumina platform. - Agency: National Institutes of Health (NIH), Center for Research on Genomics and Global Health - Purpose: Support transcriptomic research into cardiometabolic diseases - Sole source: New England Biolabs (NEB) is the only OEM specified - Products required: - NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index primer pairs), Sets 1-4 (2 units each) - NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (7 units) - NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads kit (7 units) - All products must be compatible with Illumina sequencing systems - Total sample count: 650 - No services are requested; only consumables and reagents - Period of performance: One-time use, assay completion expected within 1 year from contract award - Estimated contract value: $20,000 - $50,000 - Commercial competitors: Other molecular biology reagent manufacturers (e.g., Thermo Fisher Scientific, Qiagen, Takara Bio) could offer similar products, but NEB is specified as sole source - Competing products: Illumina RNA library prep kits, Thermo Fisher RNA library prep solutions, Qiagen RNA sequencing kits, Takara Bio RNA library prep kits

Description

The primary purpose of this acquisition is to obtain consumables and reagents essential for processing 650 plasma exosomal RNA samples for next generation sequencing. The Center for Research on Genomics and Global employs various molecular biology techniques to investigate the pathophysiology of cardiometabolic diseases and associated traits across diverse populations. This project aims to characterize the gene expression profile of plasma exosomes, which are implicated in several diseases. Plasma, a biological fluid collected via minimally invasive methods, serves as an indicator of health and disease status. Molecular analysis of plasma may further elucidate disease mechanisms and facilitate the discovery of novel biomarkers. Consumables and reagents supplied by New England Biolabs (NEB) are utilized in generating all necessary products for sequencing on the Illumina platform, the system currently available to NHGRI investigators through the core laboratory supporting this ongoing project. 

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