SAM #1232SA26Q1291

USDA ARS NCCCWA RNA Extraction and Illumina Sequencing Services for Rainbow Trout

The USDA Agricultural Research Service (ARS) National Center for Cool and Cold-Water Aquaculture (NCCCWA) is seeking quotations for specialized RNA extraction and sequencing library services for rainbow trout research. - Government Buyer: - United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Cool and Cold-Water Aquaculture (NCCCWA), ARS Acquisition and Property Division - Products/Services Requested: - RNA extraction from 90 frozen rainbow trout whole-body tissue samples - Requires quality control: RNA concentration >50ng/uL, volume >100uL, A260/A280 between 1.8-2.0, RIN >7 - RNA-seq library preparation and mRNA sequencing for 216 samples (including spleen tissue and primary muscle cell cultures) - Must use Illumina technology, paired-end 150 cycle reads, minimum 40 million reads per sample, >85% bases at QC30 quality - Return of all remaining total RNA samples after processing, shipped overnight on dry ice - Unique/Notable Requirements: - Sequencing must be performed using Illumina platforms - All deliverables (sequencing data and final report) must be completed and returned within 60 days of receiving all samples - All samples provided frozen; strict quality and labeling requirements for sample handling and return - Small business set-aside under NAICS 541380 - No specific OEMs or vendors are named in the solicitation, but Illumina is specified as the required sequencing technology

Description

This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Part 12.  This announcement constitutes the only solicitation.  Quotations are being requested and a separate written solicitation will not be issued.

Solicitation number 1232SA26Q1291 is issued as a Request for Quotation (RFQ) to conduct RNA Extraction and Sequencing Library of Rainbow Trout.

This acquisition is set-aside for small business concerns.  The applicable North American Industry Classification Standard Code is 541380.  The small business size standard is $19M.  This acquisition is a Total Small Business Set-Aside.  All responsible sources may submit a quotation which will be considered by the agency. 

See Attachment 1232SA26Q1291 for applicable clauses, instructions, and evaluation criteria.

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