SAM #140G0326Q0189

USGS Western Fisheries Research Center eDNA Metabarcoding Analysis Services for Yakima River Samples

The U.S. Geological Survey (USGS) Western Fisheries Research Center is seeking laboratory services for eDNA metabarcoding analysis of environmental samples from the Yakima River, Washington. - Government Buyer: - USGS, Department of the Interior - Western Fisheries Research Center - Services Requested: - DNA extraction on 80 environmental samples (MCE filters) - PCR amplification using universal fish primers and custom lamprey primers - Library preparation with sequencing adapters and index sequences - Next-generation sequencing (NGS) for species identification, including lamprey - Bioinformatics processing and taxonomic assignment based on sequence similarity - Final written report summarizing methods, results, and primer references; data delivered in Excel format - Quantities: - 80 samples for each laboratory step (extraction, amplification, sequencing, bioinformatics) - 1 final report - Unique Requirements: - Use of both universal fish and custom lamprey primers - Identification of lamprey species in the Yakima River basin - Delivery of report and data suitable for USGS data release - No specific OEMs or commercial vendors are named; opportunity is open to qualified laboratory service providers - Respondents must demonstrate capability to perform all steps as outlined in the statement of work - Period of performance: Final report due approximately 45 weeks after sample receipt

Description

Request for InformationeDNA Metabarcoding AnalysisThe U.S. Geological Survey (USGS) is conducting market research to determine the availability of a qualified business that can provide eDNA metabarcoding analysis in accordance with the attached statement of work. This sources sought announcement is not a request for quote or proposal and the Government is not committed to award a purchase order or contract pursuant to this announcement. The information resulting from this market research is simply for planning purposes to assist the Government in determining its acquisition strategy. The Government will not pay for any costs incurred in the preparation of information for responding to this notice.The North American Industry Classification System (NAICS) code NAICS 541380 Testing Laboratories and Services, associated size standard of $19 million applies to this announcement. All responsible sources may submit a capability statement with and relevant literature detailing the ability of fully meeting the requirement in accordance with the attached statement of work included with this announcement. All responses shall be limited to 10 pages.Responses to this announcement shall only be accepted through electronic mail addressed to Contracting Officer Joahnne Ongjoco at Joahnne_Ongjoco@ios.doi.gov and must be uploaded and received in their entirety no later than August 5, 2026 at 1700 hours Eastern Time. Responses submitted by hardcopy, or the SAM web portal shall not be accepted or considered.

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