SAM #1232SA26Q115

Rainbow Trout Whole Genome Sequencing Services for USDA ARS NCCCWA

The USDA Agricultural Research Service (ARS) National Center for Cool and Cold-Water Aquaculture (NCCCWA) is soliciting whole genome sequencing services for rainbow trout blood samples. - Government Buyer: - USDA Agricultural Research Service (ARS), National Center for Cool and Cold-Water Aquaculture (NCCCWA), ARS Acquisition and Property Division - Products and Services Requested: - High molecular weight DNA extraction from 3 frozen rainbow trout blood samples - Preparation of 6 PacBio CCSExpress libraries and sizing by Blue Pippin - PacBio Revio CCS sequencing (9 runs) to generate HiFi reads - Construction of 3 unamplified Illumina fragment libraries - Library construction using 3 Dovetail Hi-C kits (or similar) - Illumina NovaSeq X sequencing for 3 unamplified fragment libraries and 3 Hi-C libraries - Shipping and handling of samples and data delivery materials - OEMs Highlighted: - Pacific Biosciences (PacBio) for CCSExpress library prep and Revio CCS sequencing - Illumina for NovaSeq X sequencing platforms - Dovetail Genomics for Hi-C library kits - Notable Requirements: - Emphasis on high-quality DNA extraction and sequencing - Data delivery in standard formats - All deliverables required within 60 days of sample receipt - Quality control and documentation required - Quantities: - 3 DNA extractions, 6 PacBio libraries, 9 PacBio sequencing runs, 3 Illumina libraries, 3 Hi-C libraries, 3 NovaSeq X sequencing runs, 1 shipping/handling

Description

Description -

 This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Part 12.  This announcement constitutes the only solicitation.  Quotations are being requested and a separate written solicitation will not be issued.

 Solicitation number 1232SA26Q1115 is issued as a Request for Quotation (RFQ) to conduct Genome sequencing in rainbow trout.

 This acquisition  is set-aside for small business concerns.  The applicable North American Industry Classification Standard Code is 541380.  The small business size standard is $19M.  This acquisition is a Total Small Business Set-Aside.  All responsible sources may submit a quotation which will be considered by the agency. 

 See attachment “C04_Issued Solicitation_1232SA26Q1115” for applicable clauses, instructions, and evaluation criteria.

 In order to be considered for award OFFERORS SHALL SUBMIT ALL OF THE FOLLOWING:

Submit Firm Fixed Price quotation (quote on company letterhead) detailing the applicable item(s), description, price, PLACE of MANUFACTURE, and inclusive of ALL costs including , but not limited to shipping, and valid for at least 60 days after receipt of quote. 

Submit attached SF-1449 with blocks 17a, 30a, 30b, 30c, and individual Contract Line Items priced in full. 

Submit Sam.gov Unique Entity ID (UEI) with quotation. 

Submit Capability Statement and/or technical documentation/brochures showing technical capability to meet or exceed ALL salient characteristics in the Specifications.

 REJECTION OF QUOTATION: Failure to demonstrate compliance or to submit ALL items requested in solicitation prior to due date will be cause to reject the quote without further discussions.

 DISCUSSIONS: The Government intends to award without discussions but reserves the right to conduct discussions should it be deemed in the Government's best interest.

 Quotes are due on 08/14/2026 at 9:00 AM CST by email to Katelyn Nelson at katelyn.nelson@usda.gov. You must include the solicitation Title Name and Number as the subject line of the email.

Questions-All questions must go to Katelyn Nelson, whom may be reached at Katelyn.nelson@usda.gov for specific questions regarding this combined solicitation. You must include the solicitation name AND number as the subject line of the email. Questions by interested parties regarding the requirement must be submitted by 08/05/2026 by 9:00 AM CST.

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