SAM #BAC260012

Solicitation for Custom Primers and Probes for Travelers' Diarrhea Study

This opportunity is a solicitation from the Naval Medical Research Center (NAMRU SOUTH) for the supply of specialized primers and probes to support the Global Travelers Diarrhea Study. - Agency: Naval Medical Research Center (NAMRU SOUTH), Department of Defense - Purpose: Procurement of various oligonucleotide primers and probes for laboratory research in epidemiology - Products: Over 40 unique primers and probes, each specified by nucleotide sequence and required in quantities of 2 or 4 units - Examples include HLT-F, HLT-R, LT-pro, STh-F, STh-R, STh-pro, STp-F, STp-R, STp-pro, virB-F, virB-R, virB-pro, and many others - No specific OEMs, brands, or part numbers are provided; all requirements are defined by sequence and quantity - Place of performance: Bellavista, Callao, Peru - No services or installation required; this is a supply-only procurement - Intended for use in laboratory research related to travelers' diarrhea - Commercial opportunity for suppliers of custom oligonucleotide synthesis and laboratory reagents

Description

NAMRU SOUTH requests approval to award the purchase of primers and probes for protocol “The Global Travelers Diarrhea (GTD) Study: an evaluation of study design and laboratory methodologies standardization in a multisite protocol assessing the epidemiology and etiology of acquired diarrhea among a U.S. military or civilian travelers.

3. Description of Supplies or Services: 2 units HLT-F– TTC CCA CCG GAT CAC CAA  2 units HLT-R– CAA CCT TGT GGT GCA TGA TGA 2 units LT-pro – 5 ATTO550N/CTT GGA GAG AAG AAC CCT /MGBNFQ 2 units STh -F– GCTAAACCAGYAGRGTCTTCAAAA 2 units STh -R– CCCGGTACARGCAGGATTACAACA 2 units STh -pro – 5SUN-TGGTCCTGAAAGCATGAA-3MGBMGBNFQ 2 units STp -F – TGAATCACTTGACTCTTCAAAA 2 units STp -R – GGCAGGATTACAACAAAGTT 2 units STp -pro – 56FAM-TGAACAACACATTTTACTGCT-3MGB-NFQ 2 units virB-F – GGA TTT GTG CAA CGA CTT GTT AAG 2 units virB-R– GAG GAA TCT TGG CTT TGA TAA AGG 2 units virB-pro – /56FAM/ CAC TCC ATT CTG GTA ATA A /MGBNFQ/ 2 units aggR-333F– CAG CGA TAC ATT AAG ACG CCT AAA G 2 units aggR-448 R– CGT CAG CAT CAG CTA CAA TTA TTC C 2 units aggR-pro – /FAM/ CTT GCA GTT GTC CGA ATT /MGBNFQ/ 2 units eae-F– CCG ATT CCT CTG GTG ACG A 2 units eae-R– CCA CGG TTT ATC AAA CTG ATA ACG 2 units eae-pro –5 ATTO550N/iCY3/ CGT CAT GGT ACG GGT AA /MGBNFQ/ 4 units bfpA-Fw -GGT CTG TCT TTG ATT GAA TCT GCA 4 units bfpA-Rv-GCA GAC TGG TAG TAA AAC ATC ACA CC 4 units bfpA-pro-/TexasRed/ GCG CTT GCT GCC ACC GTT ACC G /3BHQ_2/ 2 units Stx1-414 F– ACT TCT CGA CTG CAA AGA CGT ATG 2 units Stx1-545 R – ACA AAT TAT CCC CTG AGC CAC TAT C 2 units Stx1-pro – /56FAM/ CTC TGC AAT AGG TAC TCC A /MGBNFQ/ 2 units HStx2-F– CCA CAT CGG TGT CTG TTA TTA ACC 2 units HStx2-R – GGT CAA AAC GCG CCT GAT AG 2 units Stx2-pro - /5SUN/ TTG CTG TGG ATA TAC GAG G /3MGBNFQ/ 4 units GFP-F-AGA TGA CGG GAA CTA CAA G 4 units GFP-Rv-CCT TCA GCT CGA TTC TAT T 4 units GFP-pro-/5Cy5/CA CCT TCG AAC TTG ACT TCA GCG C/3IAbRQSp/ 4 units Blocks Gene fragment-TTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATCTTTCAAAGATGACGGGAaCTACAAGACA CGcGCTGAAGTCAAGTTcGAAGGTGATACCCTTGTTAATAGAATCGAGcTgAAgGGTATTGATTTTAAAGAAGATGGA AACATTCTTGGACACAAATTGGAATACAACTTTAACTCACACAATGTATACATCACGGCAGACA 2 units lacY-F- ACCAGACCCAGCACCAGATAAG 2 units lacY-R -CTGCTTCTTTAAGCAACTGGCGA 2 units lacY-DLO-RFB-5 (3)-pro-FAM/CA TAC ATA TTG CCC GCC AGT ACA GAC /3BHQ_1 (3) 2 units uidA-F-GTGTGATATCTACCCGCTTCGC 2 units uidA-R - AGAACGGTTTGTGGTTAATCAGGA 2 units uidA-DLO-HB1-5-pro-HEX/TC GGC ATC CGG TCA GTG GCA GT/3BHQ_1 2 units rfc-F – ACTTATCATTGACTAGCGAGTTCT 2 units rfc-R – CGCAAGCACTATTATGAGACATTT 2 units rfc-DLO-RFB-5-pro-FAM/TG GTG TCG GTG CGA TTA TCA GTT T/3BHQ1/ 2 units wbgZ-F – CAGCGTCAGAATCGCTTACA 2 units wbgZ-R – TCAACCAGAGGCACATGTTTAT 2 units wbgZ-DLO-CAB2-5-pro-CAL Fluor Red 610/TT CAA ACG GTT TAT CAT GCG GCC G/3BHQ2/ 2 units rfpB-F – ACCAAGAAAGGGATTTCAGTCTAT 2 units rfpB- R – AAGGTTAATACCACTGCGACTT 2 units rfpB-DLO-CGB1-5-CAL Fluor™ Gold 540/AG CAG GCG CCA TTC CTG TTG ATT A/BHQ1 2 units SBO-3648-F– TTGAGCACGGAAACAGAGAG 2 units SBO-3648-R – TTCCCGTTCTTCCCTTTACAC 2 units SBO-3648-DLO-C3B2-5-pro-Cy5/TG CCG CTT CAC ATA AAT CAA CGC C/3BHQ2/ 2 units gyrA-F-CTATAACAACTGCACCTACTAAT 2 units gyrA-R-ATGAAATTTTTGCCAGTGGTG 2 units gyrA-pro-FAM/CT TAA TAG CCG TCA CCC CAC /3BHQ_1  

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