SAM #75N98026Q00841BDBIO

NIH Procurement of BD FACSMelody Cell Sorter for Diabetes Research

The National Institutes of Health (NIH), through the Office of the Director and the Office of Logistics and Operations, is seeking to procure a specialized cell sorting instrument for advanced biomedical research. - Government Buyer: - National Institutes of Health (NIH) - Office of the Director (OD) - Office of Logistics and Operations (OLAO), Office of Acquisition and Logistics Management (OALM), Hospital and Laboratory Support Division - OEM Highlight: - Becton, Dickinson and Company (BD) - Vendors Mentioned: - Becton, Dickinson and Company (BD) - Products/Services Requested: - BD FACSMelody Cell Sorter - Three lasers and nine-color capability - Quantity: 1 - No specific part number provided - Purpose: Isolate and analyze specific cell populations from organoids using fluorescent markers - Notable Requirements: - Instrument must support research on pancreatic islet organoids - Enables efficient cell population isolation and analysis for downstream experiments (cell identity, maturation, and response to experimental conditions) - Provides a cost-effective alternative to single-cell sequencing - Procurement is non-competitive and under NAICS code 334516 - Estimated contract value not to exceed $350,000

Description

THE DIABETES MILECULAR GENETICS SECTION OF THE PHOENIX EPIDEMOLOGY AND CLINICAL RESEARCH BRANCH AT THE NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES STUDIES STEM CELL DERIVED PANCREATIC ISLET ORGANOIDS COMPOSE OF MULTIPLE CELL. MANY EXPERIMENTS REQUIRE ISOLATION AND ANALYSIS OF SPECIFIC CELL POPULATIONS BECAUSE MIXED CELL POPULATIONS CAN PRODUCE UNCLEAR OR MISLEADING RESULTS. ALTHOUGH SINGLE-CELL SEQUENCING CAN IDENTIFY INDIVIDUAL CELL TYPES, IT IS COSTLY AND NOT PRACTICAL FOR ROUTING USES.  AMORE EFFICIENT APPROACH IS TO SEPARATE CELLS BASED ON PROTEINS EXPRESSED ON THE CELL SURFACE. 

THE BD FACSMELODY CELL SORTGER WITH THREE LASERS AND NINE-COLOR CAPABILITY WILL ALLOW RESEARCHERS TO ANALYZE AND ISOLATE SPECIFIC CELL POPULATIONS FROM ORGANOIDS USING FLUORESCENT MARKERS.  THIS  CAPABILITY WITH SUPPORT DOWNSTREAM EXPERIMENTS FOCUSED ON CELL IDENTITY, MATURATION, AND RESPOSE TO EXPERIMENTAL CONDITIONS WHILE REDUCING RELIANCE ON MORE EXPENSIVE SINGLE-CELL SEQUENCING METHODS

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