SAM #75N98026Q00774

Replacement Nikon TIRF Microscope System for NIH/NCI LCMB Microscopy Core Facility

The National Institutes of Health (NIH), National Cancer Institute (NCI) LCMB Microscopy Core Facility is seeking a replacement for a non-functional Nikon TIRF microscope system. - Government Buyer: - National Institutes of Health (NIH) - National Cancer Institute (NCI) - Laboratory of Cell and Molecular Biology (LCMB), Center for Cancer Research (CCR) - OEMs and Vendors: - Nikon (Ti2E Motorized PFS Microscope Bundle, Motorized E-TIRF Package, Ti2 motorized main branch and sub branch, Ti2-NS2-MU N-STORM magnification change unit) - Hamamatsu (ORCA-FusionBT sCMOS camera) - LUNF (STORM multi-line laser unit) - HP (Z4 Advanced Imaging Workstation V8) - Products/Services Requested: - Ti2E Motorized PFS Microscope Bundle (motorized encoded stage, fluorescence filter sets: DAPI, GFP, DS Red) - Nikon Motorized E-TIRF Package (includes Ti2-P-FWB-E barrier filter wheel) - Hamamatsu ORCA-FusionBT sCMOS camera (USB3.0 interface card, dual camera interface kit) - NI-BB Plus analog triggered device hub - LUNF STORM 405/488/561/640 multi-line laser unit - Ti2 motorized main branch and sub branch (exchangeable 100% reflective mirror) - C-NSTORM Quad 405/488/561/640 filter set - Ti2-NS2-MU N-STORM magnification change unit - NIS-Elements software package (6 Advanced Research licenses, HASP reconfiguration) - HP Z4 Advanced Imaging Workstation V8 (Thunderbolt 31.5-inch 4K display) - Installation kit - Services Requested: - Installation, full optical alignment, integration of new and reused components, verification of system performance to manufacturer specifications - Onsite training for LCMB Microscopy Core personnel - Unique/Notable Requirements: - Contractor must reintegrate existing components from the current instrument at no additional cost - Delivery and installation must be completed within 90–120 days after award, with installation finished within 14 days after delivery - System performance must be verified to manufacturer specifications - Onsite training is mandatory for facility personnel

Description

PRODUCT FEATURES/SALIENT CHARACTERISTICS 

The following product features and characteristics are required for this requirement: 

Ti2E Motorized PFS Microscope Bundle with motorized encoded stage and fluorescence filter sets (DAPI, GFP, DS Red) 

Nikon Motorized E-TIRF Package with Ti2-P-FWB-E barrier filter wheel 

ORCA-FusionBT sCMOS camera with USB3.0 interface card and dual camera interface kit 

NI-BB Plus analog triggered device hub 

LUNF STORM 405/488/561/640 multi-line laser unit 

Ti2 motorized main branch and sub branch with exchangeable 100% reflective mirror 

C-NSTORM Quad 405/488/561/640 filter set 

Ti2-NS2-MU N-STORM magnification change unit 

NIS-Elements software package with Advanced Research licenses (×6) and HASP reconfiguration 

Z4 Advanced Imaging Workstation V8 with Thunderbolt 31.5-inch 4K display 

Installation kit 

Reintegration of the following existing components from the current instrument at no additional cost to CCR/NCI: CFI60 Apochromat TIRF 100X Oil Immersion Objective Lens (N.A. 1.49), MCL 100 micrometer piezo nanopositioner with controller and electronics, EMCCD camera, C-NSTORM 647 filter set, C-FL TIRF CFP/YFP filter set, and NIS-Elements STORM acquisition and analysis software licenses 

DELIVERY / INSTALLATION 

Contractor shall deliver all system components within 90–120 days after award to: 

Sandeep Pallikkuth,  

LCMB, CCR, NIH/NCI  

37 Convent Dr. MSC 4256  

Building 37, Room 2033B1  

Bethesda, Maryland 20892 

Upon award and delivery, the contractor must notify the NCI Project Officer, Sandeep Pallikkuth, to schedule an installation date and time. Installation must include full optical alignment, integration of all new components, reintegration of reused components from the existing instrument, and verification of complete system performance to manufacturer specifications. The Contractor shall install the system and have it ready for use within fourteen (14) days after delivery. 

4.3 TRAINING 

Onsite training of LCMB Microscopy Core personnel on operation of the complete system is required following installation. 

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