NIH Reporter #1R21AI196856-01

NIAID Awards Grant for Research on Pseudomonas aeruginosa Exopolysaccharide Biosynthesis

This opportunity involves a research grant awarded by the National Institute of Allergy and Infectious Diseases (NIAID) to Ohio State University for studying the regulation of exopolysaccharide biosynthesis operons in Pseudomonas aeruginosa. - Government Buyer: - National Institutes of Health (NIH) - National Institute of Allergy and Infectious Diseases (NIAID) - Awardee: - Ohio State University, Department of Microbiology/Immunology/Virology - Scope of Work: - Research on post-initiation control of Pseudomonas aeruginosa exopolysaccharide biosynthesis operons (pel and psl) - Investigation of RNA chain elongation regulation and the role of Rho, NusG, and Mva proteins in transcription termination - Use of in vivo RNA probing and in vitro assays - Identification of accessory factors for processive synthesis of long EPS RNAs - Notable Requirements: - Focus on understanding mechanisms of biofilm formation and pathogenicity in P. aeruginosa - No specific OEMs, vendors, or commercial products are mentioned - Award Amount: - $439,094

Description

This project aims to investigate the regulation of RNA chain elongation in Pseudomonas aeruginosa, focusing on the pel and psl operons critical to its pathogenesis. The study will determine if Rho, NusG, and Mva proteins induce premature termination during transcription of these operons using in vivo RNA probing and in vitro assays. It will also identify accessory factors that enable the processive synthesis of long EPS RNAs, providing insights into the post-initiation control of transcription in P. aeruginosa. The research has implications for understanding biofilm formation and pathogenicity.

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