SAM #1232SA26Q0618

Chromosome Conformation and Whole Genome Sequencing Services for USDA ARS Shellfish Genetics

USDA ARS is seeking specialized sequencing services for shellfish genetics research in Kingston, RI. - Government Buyer: - United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Acquisition and Property Division (APD) - NCWMAC Shellfish Genetics program - Services Requested: - Chromosome Conformation Capture (Hi-C) library preparation and sequencing for 2 samples - DNA extraction, library preparation using a non-specific endonuclease method (e.g., OmniC kit or equivalent) - Sequencing to generate at least 100 million paired 150bp reads per sample (200 million reads total) - Data delivered as demultiplexed FASTQ files - Whole Genome Sequencing library preparation and sequencing for 21 samples - DNA extraction, library preparation, and QC - Sequencing to generate at least 100 million paired 150bp reads per sample (2.1 billion reads total) - Data delivered as demultiplexed FASTQ files - Technical Requirements: - Sequencing platform must be Illumina or equivalent - Non-biased chromosome conformation method (e.g., OmniC kit or equivalent) - Minimum 50x coverage of a 600 Mbp genome per sample - Protocol details must be provided with deliverables - Notable Details: - No specific OEMs or brands mandated, but Illumina and OmniC kit are referenced as standards - Delivery required within 60 days of sample submission; all data must be delivered by contract end date - Firm fixed price contract; early deliveries accepted - Quantities: - 2 chromosome conformation samples - 21 whole genome sequencing samples

Description

This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Part 12.  This announcement constitutes the only solicitation.  Quotations are being requested and a separate written solicitation will not be issued.  

Solicitation number 1232SA26Q0618 is issued as a Request for Quotation (RFQ) Chromosome Conformation and Whole Genome Sequencing 

This acquisition is set-aside for small business concerns.  The applicable North American Industry Classification Standard Code is 541714 The small business size standard is 1,000 employees  This acquisition is a Total Small Business Set-Aside.  All responsible sources may submit a quotation which will be considered by the agency.    

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