SAM #140G0126Q0198

USGS Solicitation for DNA Library Preparation, Oxford Nanopore Sequencing, and Bioinformatics Services

The U.S. Geological Survey (USGS) Wetland and Aquatic Research Center is seeking specialized genomic services to support environmental DNA research. - Government Buyer: - U.S. Geological Survey (USGS), Wetland and Aquatic Research Center, USGS OAG Reston Acquisition Branch - OEMs and Vendors: - Oxford Nanopore Technologies is specified as the sequencing platform manufacturer (Promethion flow cell) - No other OEMs or specific vendors are named - Products/Services Requested: - DNA library preparation for environmental DNA samples - Next generation sequencing using Oxford Nanopore Promethion flow cells - Metabarcoding analyses and protocol optimization - Bioinformatics services including sequence data trimming, quality assessment, generation of exact sequence variants, and taxonomic assignment - Processing of approximately 200-300 samples over one year - Deliverables: high-quality sequence data, Fastq files, QC reports, and output tables, all delivered electronically - Unique or Notable Requirements: - Assay optimization and 2-step PCR with high sensitivity - Super high accuracy base calling - Secure data delivery and sample handling - Services must be completed within six weeks of sample intake - Contract is set aside for small businesses, including HUBZone, SDVOSB, EDWOSB, and WOSB categories - Vendors must demonstrate technical capability and experience in similar genomic services

Description

The U.S. Geological Survey, Conservation Genetics Lab, Wetlands and Aquatic Research Center in Gainesville, FL, requires genomic services of molecular library preparations and next generation sequencing with specialized bioinformatics as described in Attachment 1 statement of work. The firm, fixed-price contract is for a one-year period.Quotes shall provide pricing, and technical capabilities and experience to perform the work. The basis of award is lowest-price technically acceptable (see E.9. Evaluation). Quote submissions are due on or before 7/02/2026. The anticipated award date is 7/16/2026.The acquisition is not a small business set-aside. The NAICS is 541380. Vendors must be registered at https://www.sam.gov/ to be awarded the contract. All responsible sources may submit a quotation which shall be considered by the government. All correspondence and quotes MUST be emailed to sruggles@usgs.gov.

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