Alaska Online Public Notices #1126277B

Alaska Department of Fish and Game Requests Wolf DNA and RNA Sequencing Services

The Alaska Department of Fish and Game, Division of Wildlife Conservation, is seeking specialized sequencing services for genetic research on free-ranging wolves: - Government Buyer: - Alaska Department of Fish and Game, Division of Wildlife Conservation, Division of Administrative Services - Products/Services Requested: - DNA isolation and whole-genome sequencing for 11 individual wolves - 150bp paired-end sequencing to ~20X depth for a 2.5GB genome (gray wolf reference GenBank GCA_905319855.2) - RNA isolation and short-read sequencing for 22 samples - ~50 million paired-end reads per sample - Long-read RNA sequencing (Iso-seq) for approximately 8 samples - High-molecular weight RNA isolation and long-read sequencing, dependent on RNA quality/quantity - All services include extraction, library preparation, and QA/QC - Notable Requirements: - Vendors must provide per-sample pricing for each assay - Ability to perform both short-read and long-read sequencing is required - Samples are primarily 6mm biopsy skin tissues stored in RNA later at -80°C; some hair or blood samples may be available - Electronic submission of quotes via email is preferred - No specific OEMs or commercial vendors are named in the solicitation - Place of Performance: - Contracting office is located in Juneau, Alaska

Description

The Alaska Department of Fish and Game is seeking RNA sequencing and whole genome sequencing services, including RNA/DNA extraction, on free-ranging wolves (Canis lupus). The procurement includes DNA isolation, whole-genome sequencing on 11 individuals, RNA isolation for short-read sequencing on 22 samples, and long-read RNA sequencing (Iso-seq) on approximately 8 samples. Electronic submission via email is preferred. The bid due date is June 8, 2026, at 2:00 PM AKST.

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