SAM #1232SA26Q0416

USDA-ARS Solicitation for Low-Coverage Whole Genome Sequencing of Rainbow Trout Samples

The USDA Agricultural Research Service (ARS) National Center for Cool and Cold Water Aquaculture (NCCCWA) is soliciting quotations for commercial services to perform genomic DNA extraction and low-coverage whole genome sequencing on 2,000 rainbow trout tissue samples. - Government Buyer: - USDA Agricultural Research Service (ARS) - National Center for Cool and Cold Water Aquaculture (NCCCWA) - Contracting Operations Branch/Acquisition and Property Division - OEMs and Vendors: - Illumina (sequencing technology required) - Products/Services Requested: - Genomic DNA extraction from 2,000 rainbow trout fin tissue samples stored in 95% ethanol - Low-coverage whole genome sequencing of 2,000 samples using Illumina technology (paired-end 150 cycles, minimum 1.2 Gbases output per sample) - Return of all unused tissue samples to the government - Delivery of sequence files in FASTQ format and a final project report - Unique/Notable Requirements: - Sequencing must be completed within 60 days of sample receipt - All deliverables (sequence files, report, unused samples) must be returned within 90 days - Samples provided by the government; contractor must supply all services, equipment, and facilities - Sequencing must use Illumina technology (genome size ~2.4Gb) - Place of Performance and Delivery: - USDA-ARS-NCCCWA, 11861 Leetown Road, Kearneysville, WV 25430 - Contracting offices in Beltsville, MD and Poplarville, MS

Description

This is a combined synopsis/solicitation for commercial service prepared in accordance with the format in FAR Part 12. This announcement constitutes the only solicitation. Quotations are being requested and a separate written solicitation will not be issued. Solicitation number 1232SA26Q0416 is issued as a Request for Quotation (RFQ) for the USDA-ARS- NEA Cool and Cold Water Aquaculture, requiring genomic DNA extractions from tissue samples stored in 95% ethanol and 0.5x whole genome sequencing via Illumina technology of 2,000 rainbow trout samples (Genome size ~2.4Gb). The Contractor shall provide all services, supplies, equipment and facilities necessary to sequence and impute genotypes in accordance with the required performance elements and benchmarks. This acquisition is set-aside for small business concerns. The applicable North American Industry Classification Standard Code is 541380 The small business size standard $19. This acquisition is a Total Small Business Set-Aside. All responsible sources may submit a quotation which will be considered by the agency. See: Attachment 1 – Statement of Work for Requirement Details

Attachment 2 – Terms and Conditions

Attachment 3-Wage Determination-2015-5415-CO_Boulder

Attachment 4-Wage Determination-2015-5613-CA_Los Angeles

Attachment 5-Wage Determination-2015-5623-CA_Almeda-Contra Costa

Attachment 6-Wage Determination-2015-5635-CA_San Diego

Attachment 7-Wage Determination-2015-5859-ID_ Jerome-Twin Falls

Attachment 8-Wage Determination-2015-6059-ME_Cumberland-Sagadahoc-York

Attachment 9-Solicitation-Request for Quote for More Solicitation Details

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