SAM #36C26226Q0653

Biodistribution Analysis of Human Spinal Cord Neural Stem Cells for VA San Diego

The Department of Veterans Affairs, Network Contracting Office 22, is seeking qualified contractors to support the NHP scNSC Biodistribution Project at the VA San Diego Healthcare System. This procurement focuses on post-mortem biodistribution analysis of human spinal cord neural stem cells (H9-scNSCs) in African green monkeys, using advanced digital droplet PCR techniques.

• Government Buyer:
  • Department of Veterans Affairs
  • Network Contracting Office 22 (NCO 22)
  • VA San Diego Healthcare System, 3350 La Jolla Village Drive, San Diego, CA 92161
• Products and Services Requested:
  • Analysis of 429 terminal African green monkey tissue samples for biodistribution
  • Method development and optimization for genomic DNA isolation from perfused samples
  • Primer design and suitability testing for human-specific sequences (HSS) by ddPCR
  • Quantification and qualification methods for H9-scNSC genomic DNA recovery rates
  • Evaluation of duplex assay inhibitory effects
  • Use of EZ connect system for gDNA isolation (429 samples)
  • Determination of H9-scNSC genome copy number using multiplex ddPCR
  • Data analysis relative to AGM reference gene TERT
  • Shipment of leftover gDNA samples to sponsor
• OEMs and Vendors:
  • No specific OEMs or vendors are named in the solicitation or attachments
• Unique or Notable Requirements:
  • Advanced molecular biology techniques (digital droplet PCR, genomic DNA isolation, primer/probe design)
  • Experience with non-human primate (NHP) tissue analysis
  • Support for IND submission preparation for clinical trials in spinal cord injury therapy
  • Set aside for Service-Disabled Veteran-Owned Small Businesses (SDVOSB)
• Place of Performance:
  • VA San Diego Healthcare System, 3350 La Jolla Village Drive, San Diego, CA 92161

Description

Stem Cell Therapy

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