SAM #75N98026Q00070

The National Institutes of Health (NIH), National Cancer Institute (NCI) Center for Cancer Research (CCR) Microscopy Core is seeking to procure a Zeiss Lattice SIM5 super-resolution light microscope system and associated supplies to support advanced cancer research imaging. - Government Buyer: - U.S. Department of Health and Human Services (HHS) - National Institutes of Health (NIH), National Cancer Institute (NCI), Center for Cancer Research (CCR), Laboratory of Cancer Biology and Genetics (LCBG), Microscopy Core - OEM Highlight: - Carl Zeiss (Carl Zeiss Microscopy, LLC) is the specified and sole OEM for all major equipment and components - Products and Supplies Requested: - Zeiss Lattice SIM5 Super-Resolution Light Microscope (part number 493505-9880-010) - Axio Observer 7 SR for Lattice SIM (409000-9810-000) - Multiple advanced modules and accessories, including: - Autoimmersion module, condensers, calibration targets and plates, joystick and stage controllers, Piezo stage attachments, stage top incubation and gas systems, universal and specialized frame inserts, holders for slides and petri dishes - High-sensitivity sCMOS cameras (e.g., ORCA Fusion BT), dual camera adapters (DuoLink SR), filter modules, laser modules (URGB, LM URGB for 405, 488, 561, 647 nm), illumination modules, and reflectors - Multiple high-performance objective lenses (C-Apo 40x/1.2 W Corr, PApo 40x/1.4 Oil DIC, Plan-Apochromat 63x/1.40 Oil DIC, LD LCI PApo 25x/0.8, PApo 20x/0.8, EC Plan-Neofluar 10x/0.30) - Scanning stages, stepper motor controls, lamphousing, illuminators, and system table (air damped, breadboard) - Microscopy Workstation Premium (HPZ8G5) with high-end processor, memory, graphics, and accessories - ZEN software suite and toolkits (3.13 DLic, Advanced Acquisition, Smart Acquisition, 2D/3D, Lattice SIM module, Hamamatsu camera driver), and annual software maintenance agreements (SMA) - ZEISS Predictive Service Remote Monitor - Services Requested: - Installation and setup by OEM-certified technician - In-person training for up to 10 users by a factory-trained specialist, to be completed within 30 business days of installation - Minimum 12-month manufacturer warranty and maintenance support - Unique/Notable Requirements: - All major components must be manufactured by Carl Zeiss - Delivery and installation required within 6 months of award; training within 2 weeks to 30 business days post-installation - Equipment must support simultaneous two-channel imaging, live cell imaging, and super-resolution imaging with both oil and water immersion objectives - Place of Performance/Delivery: - National Institutes of Health, National Cancer Institute, 37 Convent Drive, Bldg 37, Room B114A, Bethesda, MD 20892

The U.S. Department of Health and Human Services (HHS), National Institutes of Health (NIH), National Cancer Institute (NCI) Center for Cancer Research (CCR), Laboratory of Cancer Biology and Genetics (LCBG), CCR Microscopy Core requires one (1) new, commercially available, Zeiss Lattice SIM5 super-resolution light microscope.

The National Cancer Institute is the principal federal agency for cancer research and training. It leads, conducts, and supports cancer research to advance scientific knowledge and improve cancer prevention, detection, diagnosis, and survivorship. The Center for Cancer Research (CCR) is the largest division of the NCI’s intramural research program with both basic and clinical research groups exploring the cutting edge of cancer research involving a wide spectrum of biological and biomedical problems that range from visualizing and understanding the structure of genes and proteins and developing novel assays to detect and potentially treat patients. The CCR Microscopy Core, the main microscopy core in the CCR, serves the imaging needs of a wide range of NCI researchers studying the cell biology of cancer.  The core facility has five high-quality research-grade instruments, including confocal, multi-photon and super-resolution microscopes, to apply to the numerous research projects from the over 45 labs/branches within NCI, that use the Core annually. To satisfy the demands for super-resolution fluorescence microscopy, the CCR Microscopy Core requests a new Zeiss Lattice SIM5 structured illumination super-resolution microscope. Structured illumination super-resolution microscopy is an established method for acquiring high resolution images of biological samples and is in high demand from NCI researchers. The demand arises from the need to understand the molecular interactions of cellular proteins and the gene expression profiles of tumor cells. Super-resolution microscopy provides researchers the ability to visualize these molecular interactions within cells, and the newest SIM technology available from the requested Zeiss Latice SIM5 microscope will allow researchers to image these interactions in live cells. Thus, providing this cutting-edge technology to NCI researchers to apply to their cancer research projects and further improve our understanding of the cell biology of cancer.

The CCR Microscopy Core requires a new Zeiss Lattice SIM5 super-resolution microscope that shall  include a high-speed lattice SIM5 scanner, dual high-sensitivity sCMOS cameras with Duolink configuration for simultaneous 2 channel imaging, four high-power lasers (wavelengths 405, 488, 561 and 647 nm), 500 um Piezo stage, stage-top incubation for live cell imaging, and both high numerical aperture oil and water immersion objective lenses for super-resolution imaging. Thus, it provides extended capabilities to image complex samples but also has the capability to perform functional live cell SIM imaging on larger biological samples. All users of the CCR Microscopy Core interested in performing SIM super-resolution microscopy will benefit from the requested Zeiss Lattice SIM5 microscope.