Award
The University of Texas at San Antonio 743-2023-IIFB-1008
Gene modifications for iPSC cell lines
Recipient
Not Specified
Awarded
June 22, 2023
Identifier
743-2023-IIFB-1008
The University of Texas at San Antonio (UTSA) is the government buyer soliciting gene modification services for induced pluripotent stem cell (iPSC) lines. The awarded service involves removing 8 extra alanine residues on the ARX gene in patient-derived iPSC lines, specifically reducing from 20 alanines to the normal 12 in the second poly-alanine tract (PA2). The requesting department, UTSA's NDRB Department, will supply the human iPSC cells. The vendor is required to produce at least 2 clones of isogenic control lines with the alanine deletion, ensuring no off-target edits and maintaining a normal karyotype after gene editing. The turnaround time for this service is 30 weeks or less, and the vendor must demonstrate a high success rate (>70%). The solicitation includes one product line item for the gene modification service (Knockin-Fragment Deletion, Cell Clone, iPSC, User Supplied, VIAL) with a quantity of 1 service unit, and a second product line item for domestic shipping services. The primary contact for this award is Robin Davis at UTSA. The contract requires the vendor to enter into a University Purchase Order agreement under Texas Education Code 51.9335. No specific OEMs or vendors are named in the document. The place of performance is at UTSA in San Antonio, Texas.
Description
The project involves gene modifications for iPSC cell lines. The potential bidder would need to remove 8 extra alanine on ARX gene. Patient has 20 Ala in the second poly-alanine tract (PA2) instead of normal 12. The requesting department will provide human induced pluripotent stem cells (hiPSCs) derived from patients, and the bidder is asked to obtain at least 2 clones of isogenic control where the 8 extra alanine have been removed without off target edits. Isogenic controls must also have normal karyotype after gene editing. The turnaround should be 30 weeks or less. Potential bidders must have a high rate of success (>70%).