NIH Reporter #5UM1HG012649-05

Molecular and cellular characterization of essential human genes.

NIH-funded project by Northwestern University to create a catalog of null alleles for essential human genes using a chemically inducible system in human stem cells, facilitating studies of gene function in development and disease.

Description

This project aims to generate barcoded and conditional null alleles in a cellular system to model early human development and a broad range of human diseases. It involves creating a catalog of molecular and cellular phenotypes of null alleles for nearly every human gene using in vitro multicellular systems. The approach uses a chemically inducible and reversible system for rapid depletion of target proteins, enabling temporal control of protein levels to study null alleles, particularly focusing on 250 essential genes implicated in human diseases. The project is funded by NIH and organized by Northwestern University.

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