Award
National Cancer Institute 5F31CA288070-02
Regulation of the retinoblastoma (Rb) tumor suppressor by the ubiquitin-proteasome system
Recipient
University of North Carolina at Chapel Hill
Award Amount
$40,752.00
Ceiling
$40,752.00
Awarded
July 24, 2025
Identifier
5F31CA288070-02
The award supports research on the regulation of the retinoblastoma tumor suppressor by the ubiquitin-proteasome system, focusing on its degradation's role in breast cancer cell cycle control and therapeutic response.
Description
This project investigates how the ubiquitin-mediated degradation of the retinoblastoma (Rb) tumor suppressor controls cell cycle progression and therapeutic response in breast cancer. The research aims to identify the ubiquitin ligase (E3) responsible for Rb degradation and to understand how Rb degradation affects cell cycle regulation and response to Cdk4/6 inhibitors. The study involves mass spectrometry, CRISPR/Cas9 gene editing, and live-cell imaging to elucidate the timing and regulation of Rb degradation. The findings could provide insights into overcoming resistance to Cdk4/6 inhibitors and enhance understanding of Rb’s role beyond phosphorylation-dependent inactivation.