Award
NIH Reporter #1ZIABC012099-04
Interrogation of Genetic Dependencies in Renal Cell Carcinomas.
Recipient
DIVISION OF BASIC SCIENCES - NCI, UNITED STATES
Award Amount
$447,770.00
Ceiling
$447,770.00
Awarded
October 04, 2023
Identifier
1ZIABC012099-04
NIH funds a project using advanced CRISPR screening to identify and validate metabolic dependencies in renal cell carcinoma, aiming to find therapeutic targets and understand genetic modifiers affecting drug sensitivity.
Description
This NIH-funded research project focuses on identifying metabolic vulnerabilities in renal cell carcinoma using a Cas9-Cas12a CHyMErA-based combinatorial CRISPR screening platform in patient-derived RCC cell lines. The project aims to validate screen hits, understand molecular mechanisms influencing cell fitness, and assess conservation of vulnerabilities across RCC models and other cancer types, alongside chemogenetic screens to find genetic modifiers affecting sensitivity to small-molecule inhibitors.