Award
National Institute on Aging 5U01AG089040-02
Development, Validation, and Harmonization of Methods for Collection and Measurement of NAD+ and Related Metabolites: Application to a Randomized Trial of Patients Undergoing Coronary Artery Bypass
Recipient
Brigham and Women's Hospital
Award Amount
$1,213,239.00
Ceiling
$1,213,239.00
Awarded
August 15, 2025
Identifier
5U01AG089040-02
This NIH-funded project by Brigham and Women's Hospital focuses on developing and harmonizing methods to measure NAD+ and related metabolites, including a clinical trial to test oral NMN's effect on myocardial NAD+ levels during coronary artery bypass surgery.
Description
The project aims to establish robust sample collection, processing, and storage protocols that preserve the integrity of NAD+, NADH, NADP, NADPH, and related metabolites. It involves optimizing LC/MS methods for measuring these metabolites, creating commutable standards, and harmonizing assays across laboratories. The study includes a randomized, double-blind, placebo-controlled trial to assess whether oral NMN administration can increase myocardial NAD+ levels and reduce injury during coronary artery bypass graft surgery.