Award
NIH Reporter #2R15GM093330-04
Use of Vibrational Reporters to Measure Dynamics and Distances in RNA and Proteins
Recipient
Franklin and Marshall College
Award Amount
$423,133.00
Ceiling
$423,133.00
Awarded
June 06, 2024
Identifier
2R15GM093330-04
This NIH grant funds research using infrared spectroscopy with vibrational reporters to study protein and RNA dynamics, aiming to advance understanding relevant to diseases and RNA-based therapies.
Description
The proposed research focuses on using infrared (IR) spectroscopy with vibrational reporters (nitriles and azides) to probe local structure and dynamics in two proteins (adenylate kinase [AK] and superfolder green fluorescent protein) and in RNA hairpins. Mutations in AK are associated with hemolytic anemia, reticular dysgenesis, and ciliary dyskinesia. RNA structure is complex and the information gained from this research has the potential to benefit future advances in RNA vaccines, CRISPR, and RNA interference therapies.