Award
NIH Reporter #1U18FD008342-01
Validation of Endopep-MS for qualitative detection of BoNT/A, /C, /CD, /D, and /DC in animal specimens and feed.
Recipient
Not Specified
Award Amount
$49,689.00
Ceiling
$49,689.00
Awarded
July 31, 2024
Identifier
1U18FD008342-01
Award to validate Endopep-MS method for detecting botulinum neurotoxins in animal specimens and feed, comparing it to the mouse bioassay and developing antibodies for toxin extraction.
Description
This project aims to validate the Endopep-MS method for the qualitative detection of botulinum neurotoxins (BoNTs) in animal specimens and feed. The study will compare the method to the standard mouse bioassay (MBA) using toxin-spiked and archived samples. It also involves developing monoclonal antibodies for toxin extraction and assessing the effectiveness of polyclonal antibodies.