Award
National Human Genome Research Institute 5R21HG014016-02
Self-Evolvr: self-refreshing molecular barcodes using CRISPR-self-guided mutagenesis
Recipient
Harvard Medical School
Award Amount
$204,695.00
Ceiling
$204,695.00
Awarded
April 03, 2026
Identifier
5R21HG014016-02
NIH awarded a research grant to Harvard Medical School for developing Self-EvolvR, a self-refreshing DNA barcode technology using CRISPR to improve lineage tracing in biological systems, with a total award amount of $204,695.
Description
This project develops Self-EvolvR, a new self-refreshing DNA barcode technology aimed at addressing the limitations of current evolving barcode systems for high-resolution lineage tracing across various biological systems. The research focuses on the development, optimization, and validation of Self-EvolvR to enable scalable, quantitative measurements of cellular lineages over time across a large variety of organisms and systems using an engineered Cas9 system to continually introduce mutations into the barcode over long periods without self-inactivation.