Award
NIH Reporter #5R21OD037867-02
Development of Transgenic Mouse Model Resources for Red Fluorescence-Based Genetically Encoded Indicator Studies
Recipient
University of Nebraska Medical Center
Award Amount
$191,875.00
Ceiling
$191,875.00
Awarded
July 27, 2026
Identifier
5R21OD037867-02
NIH awarded a research grant to develop transgenic mouse models with red fluorescent protein-based indicators using CRISPR genome engineering to improve cellular imaging capabilities, addressing limitations of green fluorescent protein models.
Description
This project aims to develop mouse models expressing red fluorescent protein (RFP)-based indicators, characterize them, and make them available to the scientific community. The models will enable imaging of intracellular processes such as calcium activity and membrane potential, which are challenging with current green fluorescent protein (GFP)-based models due to phototoxicity and autofluorescence. The approach involves using CRISPR-based genome engineering to swap GFP for RFP in a well-studied transgenic mouse strain. The models will be suitable for crossing with Cre driver mice to study various cell types.