Award
NIH Reporter #1R21AI200041-01
Understanding the impact of human protein coding variation on STING function.
Recipient
CASE WESTERN RESERVE UNIVERSITY, CLEVELAND, OH, UNITED STATES
Award Amount
$235,500.00
Ceiling
$235,500.00
Awarded
June 16, 2026
Identifier
1R21AI200041-01
The award funds research to understand how mutations in the STING protein affect its function and contribute to inflammatory diseases, aiming to develop targeted therapies.
Description
This project aims to develop high-throughput, multiplexable assays to systematically characterize how SAVI mutations affect STING-mediated transcription, autophagy, and cell death across common human genetic backgrounds. The work includes creating a barcoded library of SAVI mutations in cis with the five most common human STING alleles, using synthetic transcriptional reporters for IRF3, NF-κB, and TFEB/TFE3 activity, and evaluating autophagy capacity via FACS-based GFP-LC3 puncta formation assay. The goal is to understand how variants modulate STING’s functions and to inform targeted therapeutic strategies for STING-related disorders.