Award
National Heart, Lung, and Blood Institute 5R01HL161361-04
CRISPR-based transistors for high throughput multiplexed monitoring of CRISPR-based editing efficiency for Sickle cell disease
Recipient
University of California, San Diego
Award Amount
$431,340.00
Ceiling
$431,340.00
Awarded
March 03, 2025
Identifier
5R01HL161361-04
Development of a novel electronic platform utilizing multiplex CRISPR-transistors to quantify specific DNA sequences directly from unamplified genomic DNA to improve gene therapy quality control for Sickle Cell Disease.
Description
This project aims to develop a platform using multiplex CRISPR-transistors to rapidly assess and optimize CRISPR/Cas9 editing efficiency in Sickle Cell Disease gene therapy. The platform will electronically detect single nucleotide changes without amplification, providing quick and reliable quality control for gene therapy.