Award
National Institute of General Medical Sciences 5R01GM145886-05
Probing conformational changes by protein surface azidation
Recipient
University of Illinois at Chicago
Award Amount
$335,790.00
Ceiling
$335,790.00
Awarded
April 15, 2025
Identifier
5R01GM145886-05
This NIH-funded project at University of Illinois at Chicago develops a chemoproteomic platform to study protein conformational changes and interactions in live cells, advancing molecular biology research.
Description
Using azide-containing hypervalent iodine reagents, this project develops a chemoproteomic platform called Protein Surface Azidation Mass Spectrometry (ProSurA-MS) to detect conformational changes on protein surfaces, map protein dynamics, and study protein-protein interactions in live cells. The project aims to characterize conformational changes induced by post-translational modifications and metal binding, understand the chemical mechanisms behind ProSurA, develop improved reagents, and establish methods for identifying protein-protein interactions in live cells.