Award
National Institute of General Medical Sciences 1R15GM154315-01
Improved Understanding of Bacterial Sortase Activity Towards Next-Generation Protein Engineering
Recipient
Western Washington University
Award Amount
$382,912.00
Ceiling
$382,912.00
Awarded
March 29, 2024
Identifier
1R15GM154315-01
NIH grant awarded to Western Washington University to study bacterial sortase enzymes for protein engineering and antibiotic development, with a focus on understanding enzyme selectivity and activity.
Description
This project aims to dissect the selectivity and activity determinants of bacterial sortase enzymes from multiple classes, including the transmembrane regions of sortases and their substrates. It will develop high-throughput bacterial display assays to measure sortase selectivity across all classes, utilizing protein biochemistry, structural biology, chemical biology, and computational biology.