Award
NIH Reporter #5R01GM147960-04
Mechanisms of replication origin licensing studied by real-time single-molecule fluorescence
Recipient
Massachusetts Institute of Technology, Cambridge, MA
Award Amount
$384,301.00
Ceiling
$384,301.00
Awarded
June 06, 2025
Identifier
5R01GM147960-04
This NIH-funded project aims to elucidate the mechanisms of DNA replication origin licensing using advanced single-molecule fluorescence techniques and molecular genetics, focusing on helicase loading and the role of the origin recognition complex in eukaryotic cells.
Description
The project investigates the mechanisms of replication origin licensing, focusing on the role of origin recognition complex (ORC) and helicase loading in eukaryotic DNA replication. It employs single-molecule fluorescence microscopy and molecular genetics to understand the dynamic processes involved.