Award
National Institute on Aging 1R21AG086744-01A1
SASP-ER platform to identify spatiotemporal senescence-associated secretome
Recipient
University of Pittsburgh at Pittsburgh
Award Amount
$423,179.00
Ceiling
$423,179.00
Awarded
November 14, 2024
Identifier
1R21AG086744-01A1
This NIH-funded project aims to develop a novel SASP-ER platform to identify and analyze the senescence-associated secretory phenotype in vivo, enabling better understanding and diagnostic testing of age-related diseases.
Description
The goal of this R21 project is to build a research platform to assess senescence-associated secretory phenotype (SASP) in vivo. A prominent feature of cellular senescence is the secretion of diverse biologic factors (cytokines, chemokines, proteases etc), collectively referred to as the SASP. The SASP is hypothesized to be a key player in driving age-related diseases. However, SASP has been characterized almost exclusively in vitro due to the challenge of identifying the source of a secreted protein in vivo. This project aims to combine an innovative research infrastructure platform to assess SASP and multiple aging paradigms to elucidate the molecular identity of the SASP-secretome in vivo, using a novel SASP-ER platform that permits the conditional tagging of secreted proteins in specific cell types.