NIH Reporter #1ZICHG200349-18

NHGRI/DIR Embryonic Stem Cell and Transgenic Mouse Core

This award funds the NHGRI Embryonic Stem Cell and Transgenic Mouse Core, which provides advanced services for generating genetically engineered mice using CRISPR/Cas and other methods, supporting NHGRI investigators in genetic research.

Description

The NHGRI Embryonic Stem Cell and Transgenic Mouse Core provides a shared service to NHGRI investigators specializing in generating genetically engineered mice via conventional transgenesis, ES cell targeting, and primarily genome editing using CRISPR/Cas. The Core breeds 90% of animals in-house, utilizes several background strains, maintains a colony of Cre expressing and other transgenic mice, cryopreserves important mice, and supports investigators in gene targeting/editing and transgenesis. Services include teratoma analysis, embryo harvest, fibroblast isolation, and embryonic stem cell generation. The Core is at the forefront of transgenic production using CRISPR/Cas and has generated targeted transgenics for hundreds of constructs since 2013.

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