Award
NIH Reporter #1F31AI194803-01
Investigating structural and biochemical factors guiding concanamycin A biosynthesis
Recipient
University of Michigan at Ann Arbor
Award Amount
$43,588.00
Ceiling
$43,588.00
Awarded
June 23, 2025
Identifier
1F31AI194803-01
This award funds research to investigate the structural and biochemical factors guiding the biosynthesis of concanamycin A, a natural product with potential therapeutic applications such as HIV treatment. The project uses advanced structural biology and proteomics techniques to understand the synthesis and storage of CMA.
Description
Concanamycin A (CMA) is a polyketide natural product with potential therapeutic applications, including HIV treatment. The project aims to elucidate the structure of the cellular machinery synthesizing CMA and understand its intracellular storage mechanisms. This involves in situ structural analysis of the PKS complex using cryo-electron tomography, cross-linking mass spectrometry, and integrative modeling. Additionally, the project will identify proteins involved in CMA storage and their roles in CMA biosynthesis, using proteomics and infrared nanospectroscopy.