Award
NIH Reporter #1R16GM159693-01
Mechanisms, Structures and Engineering of Flavonoid-producing Dioxygenase Enzymes
Recipient
CALIFORNIA STATE UNIV SAN BERNARDINO
Award Amount
$147,000.00
Ceiling
$147,000.00
Awarded
September 01, 2025
Identifier
1R16GM159693-01
Research grant to study mechanisms and structures of flavonoid-producing dioxygenase enzymes to support enzyme engineering and biosynthesis for health research.
Description
The research aims to enhance understanding of alpha ketoglutarate-dependent dioxygenases, focusing on enzymes Flavanone Synthase 1 (FLS1) and Flavanone 3-Hydroxylase (F3H). These enzymes produce the medically significant compounds quercetin and trans-taxifolin, respectively. The project will employ high-resolution mass spectrometry, isotope labeling, and X-ray crystallography to elucidate enzyme mechanisms and structures, supporting enzyme engineering and biosynthesis of flavonoids for health research.