Award
National Institute of General Medical Sciences 5R35GM118129-10
Mechanisms of DNA replication and maintenance in eukaryotes
Recipient
Washington University
Award Amount
$697,356.00
Ceiling
$697,356.00
Awarded
May 30, 2025
Identifier
5R35GM118129-10
This NIH-funded project investigates DNA replication and maintenance mechanisms in eukaryotes, focusing on key proteins and pathways relevant to genetic stability and cancer research.
Description
Research focusing on the mechanisms of DNA replication in eukaryotic cells, mutagenesis due to replication dysfunction, and cell cycle checkpoint activation using biochemical, biophysical, and genetic analyses in Saccharomyces cerevisiae, with structural studies of the complex machineries involved. Key proteins studied include DNA polymerase z, Rev1, Mec1, and Tel1 (orthologs of human ATR and ATM). The project aims to unravel the dual regulatory functions of Rev1 and understand how kinase activities of Mec1 and Tel1 are activated, with implications for understanding DNA damage response pathways.