National Institute on Aging 5K18AG095816-02

Developing a Comparative Single-Cell Spatial Transciptomic Atlas of Brain Aging in Non-Human and Humans

Award to Tulane University of Louisiana to develop a comparative single-cell spatial transcriptomic atlas of brain aging in humans and rhesus macaques, aiming to identify conserved gene expression changes related to neurodegeneration and provide advanced training in neurobiological research methods.

Description

This project aims to develop the first lifespan-matched, comparative single-cell spatial transcriptomic atlas of the prefrontal cortex and hippocampus in humans and rhesus macaques. Using the CosMx Spatial Molecular Imager (SMI), high-resolution, single-cell gene expression profiles will be generated across different life stages—young, adult, and aged—in both species. The central hypothesis is that aging induces specific, identifiable spatial and cell-type-specific gene expression alterations conserved between humans and rhesus macaques, contributing to neurodegeneration. Under the guidance of expert mentors, the candidate will receive comprehensive training in NHP aging models, advanced spatial transcriptomics, human neuropathology, data interpretation, and cross-species comparative analysis using integrative bioinformatics. This research will fill a critical gap in understanding brain aging and may inform therapeutic strategies to mitigate neurodegenerative diseases.

View original record