NIH Reporter #1R01HG014170-01A1

High-throughput and accessible single-cell multiomic technologies for DNAmethylation analysis

This award funds the development of innovative single-cell DNA methylation analysis technologies to improve understanding of gene regulation and disease mechanisms, using advanced sequencing and library construction methods.

Description

The project aims to develop new technologies for single-cell assessment of DNA methylation, enabling high-throughput, cost-effective, and precise analysis across development and disease research disciplines. It proposes to leverage cutting-edge cell encapsulation, transposase-based library construction, and nanopore sequencing to develop a platform for direct detection of DNA methylation in previously inaccessible genomic regions, including repetitive regions and allele-specific methylation patterns. The technologies will be demonstrated on diverse samples including human PBMCs, cerebral cortex, induced pluripotent stem cells, and control cell lines.

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