National Institute of Allergy and Infectious Diseases 5R01AI178848-04

Development and application of a quantitive model for HIV-1 transcriptional activation driven by TAR RNA conformational dynamics

This NIH grant supports research to develop predictive models of HIV-1 transcriptional activation by studying RNA conformational dynamics and RNA-protein interactions, aiming to improve understanding of HIV replication and therapeutic interventions.

Description

The proposed research aims to develop a predictive molecular understanding of HIV-1 replication processes, focusing on RNA-protein interactions and RNA structural dynamics. The project involves creating technologies for high-throughput data collection, integrating NMR data with computational models, and developing thermodynamic models to predict cellular transcriptional activity based on TAR RNA sequences. The research will generate atomic-resolution ensembles for TAR variants, refine conformational propensities, and extend models to include small molecule interactions and RNA competition, ultimately enabling quantitative predictions of HIV transcriptional activity from RNA sequences.

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