Award

National Eye Institute (NEI), National Institute of Neurological Disorders and Stroke (NINDS) 1R21EY038741-01

High-Throughput Computational Pipeline for Rapid Development of Photocaged Peptide Tools

Recipient

Utah State Higher Education System--University of Utah

Award Amount

$616,000.00

Ceiling

$616,000.00

Awarded

July 20, 2026

Identifier

1R21EY038741-01

Development of an open-source computational pipeline for designing photocaged neuropeptides to enable precise light-induced activation, reducing development time and costs.

Description

This project aims to develop a generalizable, open-source computational machine learning pipeline for the rapid, rational design of photocaged neuropeptides. The pipeline will integrate solvent-accessibility mapping, receptor docking, and molecular dynamics simulations leveraging experimentally resolved structures and AI-predicted AlphaFold2 models. The goal is to generate neuropeptide analogs that prevent GPCR binding in darkness but restore activity after photolysis, enabling precise light-induced activation. The project will synthesize and validate top analogs through high-throughput assays and electrophysiological studies, significantly reducing peptide development timelines and costs.

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