Award
National Institute on Aging 4R00AG075242-03
Synthetic Reconstruction of Human Chaperone Networks in Yeast Models of Neurodegeneration
Recipient
Temple University of the Commonwealth
Award Amount
$249,000.00
Ceiling
$249,000.00
Awarded
May 19, 2026
Identifier
4R00AG075242-03
This NIH-funded project at Temple University aims to map human chaperone networks in yeast and human cell models to identify therapeutic targets for neurodegenerative diseases associated with protein aggregation.
Description
The project aims to build the first exhaustive map of a chaperone network against ADRD-associated proteins TDP43, FUS, and TAU. Using a novel genetic technique, all 12,155 possible combinations of the human Hsp40-Hsp70-Hsp110 network will be expressed and studied in yeast models. The active chaperones will be further analyzed in human cells and in vitro assays to assess their ability to prevent and reverse TDP43 aggregation. The project will also expand to study TAU aggregation and develop a system for overexpressing all 194 human chaperones in human cell models to identify therapeutic targets for ADRD.